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Abmart Inc p-insr thr1375
Antibodies (human and mouse) used in this study
P Insr Thr1375, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Antibodies (human and mouse) used in this study

Journal: Journal of Translational Medicine

Article Title: VEGFB ameliorates insulin resistance in NAFLD via the PI3K/AKT signal pathway

doi: 10.1186/s12967-024-05621-w

Figure Lengend Snippet: Antibodies (human and mouse) used in this study

Article Snippet: p-INSR Thr1375 , 1:1000 , Rabbit , Abmart.

Techniques:

VEGFB activates PI3K/AKT signal pathway in insulin-resistant HepG2 cells ( A ) Intracellular glucose content ( n = 3) ( B ) Intracellular glycogen content ( n = 3) ( C ) PPI analysis ( D ) VEGFR1, INSR, p-INSR Thr1375 , IRS1, p-IRS1 Ser307 , PI3K, p-PI3K Tyr467 , AKT, p-AKT Ser473 , GLUT2, GSK3β, p-GSK3β Ser9 , FOXO1, and p-FOXO1 pS256 proteins expression were detected by western blot ( E ) VEGFR1/β-actin expression ( n = 3) ( F ) p-INSR Thr1375 /INSR expression ( n = 3) ( G ) p-IRS1 Ser307 /IRS1 expression ( n = 3) ( H ) p-PI3K Tyr467 /PI3K expression ( n = 3) ( I ) p-AKT Ser473 /AKT expression ( n = 3) ( J ) GLUT2/β-actin expression ( n = 3) ( K ) p-GSK3β Ser9 /GSK3β expression ( n = 3) ( L ) p-FOXO1 pS256 /FOXO1 expression ( n = 3) ( M ) Effects of VEGFB on VEGFR1, INSR, IRS1, PI3K, AKT, GLUT2, G6Pase, and PEPCK genes expression at mRNA level ( n = 3) * indicates to compare with HepG2 group, # indicates to compare with IR + siRNA-NC group, Δ indicates to compare with IR + LV-NC group with a significant difference p < 0.05

Journal: Journal of Translational Medicine

Article Title: VEGFB ameliorates insulin resistance in NAFLD via the PI3K/AKT signal pathway

doi: 10.1186/s12967-024-05621-w

Figure Lengend Snippet: VEGFB activates PI3K/AKT signal pathway in insulin-resistant HepG2 cells ( A ) Intracellular glucose content ( n = 3) ( B ) Intracellular glycogen content ( n = 3) ( C ) PPI analysis ( D ) VEGFR1, INSR, p-INSR Thr1375 , IRS1, p-IRS1 Ser307 , PI3K, p-PI3K Tyr467 , AKT, p-AKT Ser473 , GLUT2, GSK3β, p-GSK3β Ser9 , FOXO1, and p-FOXO1 pS256 proteins expression were detected by western blot ( E ) VEGFR1/β-actin expression ( n = 3) ( F ) p-INSR Thr1375 /INSR expression ( n = 3) ( G ) p-IRS1 Ser307 /IRS1 expression ( n = 3) ( H ) p-PI3K Tyr467 /PI3K expression ( n = 3) ( I ) p-AKT Ser473 /AKT expression ( n = 3) ( J ) GLUT2/β-actin expression ( n = 3) ( K ) p-GSK3β Ser9 /GSK3β expression ( n = 3) ( L ) p-FOXO1 pS256 /FOXO1 expression ( n = 3) ( M ) Effects of VEGFB on VEGFR1, INSR, IRS1, PI3K, AKT, GLUT2, G6Pase, and PEPCK genes expression at mRNA level ( n = 3) * indicates to compare with HepG2 group, # indicates to compare with IR + siRNA-NC group, Δ indicates to compare with IR + LV-NC group with a significant difference p < 0.05

Article Snippet: p-INSR Thr1375 , 1:1000 , Rabbit , Abmart.

Techniques: Expressing, Western Blot

Effects of VEGFB on hepatic insulin resistance via PI3K/AKT signal pathway ( A ) VEGFR1, INSR, p-INSR Thr1375 , IRS1, p-IRS1 Ser307 , PI3K, p-PI3K Tyr467 , AKT, p-AKT Ser473 , GLUT2, GSK3β, p-GSK3β Ser9 , FOXO1, and p-FOXO1 pS256 proteins expression ( B ) VEGFR1/β-actin expression ( C ) p-INSR Thr1375 /INSR expression ( D ) p-IRS1 Ser307 /IRS1 expression ( E ) p-PI3K Tyr467 /PI3K expression ( F ) p-AKT Ser473 /AKT expression ( G ) GLUT2/β-actin expression ( H ) p-GSK3β Ser9 /GSK3β expression ( I ) p-FOXO1 pS256 /FOXO1 expression ( J ) Effects of VEGFB on VEGFR1, IRS1, PI3K, AKT, GLUT2, INSR, PEPCK, and G6Pase genes expression at mRNA level ( K ) Hepatic glucose content ( L ) Hepatic glycogen content * indicates to compare with SD group, # indicates to compare with HFD-WT group, Δ indicates to compare with HFD-KO group with a significant difference p < 0.05

Journal: Journal of Translational Medicine

Article Title: VEGFB ameliorates insulin resistance in NAFLD via the PI3K/AKT signal pathway

doi: 10.1186/s12967-024-05621-w

Figure Lengend Snippet: Effects of VEGFB on hepatic insulin resistance via PI3K/AKT signal pathway ( A ) VEGFR1, INSR, p-INSR Thr1375 , IRS1, p-IRS1 Ser307 , PI3K, p-PI3K Tyr467 , AKT, p-AKT Ser473 , GLUT2, GSK3β, p-GSK3β Ser9 , FOXO1, and p-FOXO1 pS256 proteins expression ( B ) VEGFR1/β-actin expression ( C ) p-INSR Thr1375 /INSR expression ( D ) p-IRS1 Ser307 /IRS1 expression ( E ) p-PI3K Tyr467 /PI3K expression ( F ) p-AKT Ser473 /AKT expression ( G ) GLUT2/β-actin expression ( H ) p-GSK3β Ser9 /GSK3β expression ( I ) p-FOXO1 pS256 /FOXO1 expression ( J ) Effects of VEGFB on VEGFR1, IRS1, PI3K, AKT, GLUT2, INSR, PEPCK, and G6Pase genes expression at mRNA level ( K ) Hepatic glucose content ( L ) Hepatic glycogen content * indicates to compare with SD group, # indicates to compare with HFD-WT group, Δ indicates to compare with HFD-KO group with a significant difference p < 0.05

Article Snippet: p-INSR Thr1375 , 1:1000 , Rabbit , Abmart.

Techniques: Expressing